Why is it important to collect a discard tube before collecting your blood sample?
The discard tube must be used to prime the tubing of the collection set, which will assure maintenance of the proper anticoagulant/blood ratio in the first tube filled. The discard tube should be a non-additive or a coagulation tube, and need not be completely filled.
What can be used as a discard tube?
discard tube prior to coagulation collection. Another citrate (blue top) tube should be used as the discard tube. 3. Serum – Inside walls of tube are coated with a clot activator to promote coagulation.
When collecting blood which tube is collected first?
The order of draw is based on CLSI Procedures and Devices for the Collection of Capillary Blood Specimens; Approved Standard – Sixth Edition, September 2008. This standard recommends that EDTA tubes be drawn first to ensure good quality specimen, followed by other additive tubes and finally, serum specimen tubes.
What color is a discard tube?
| 1. | Blood Cultures |
|---|---|
| 2. | Discard tube 3 mL “no additive” must be used ONLY if drawing a citrate (blue) tube with a tube holder and butterfly needle. Used only to remove air from tubing. |
| 3. | Light Blue top tube 1.8 mL (Na Citrate) Light Blue top tube 2.7 mL (Na Citrate) |
| 4. | Red top tube 5 mL (Clot Activator) |
What is discard tube?
Discard tubes do not contain any additives, but are evacuated and sterile on the inside. If blood is drawn, for example with a (safety) blood collection set, it is recommendable to use a discard tube to displace any air in the tubing of the winged needle prior to filling other tubes.
When should a discard tube be drawn during venipuncture?
When coagulation tests are performed, the recommended guideline is that a discard tube be used and the coagulation testing should be done only on the second tube. This is done as the contamination in the first tube by tissue thromboplastin released during the venipuncture may affect the test result.
What color tube should be collected for coagulation studies?
blue
Sample Collection for Coagulation Testing. 1. Collection Tube. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.
What is the light blue tube used for in phlebotomy?
These tubes contain the anticoagulant sodium citrate. They are used mostly for coagulation (clotting) studies. They must be completely filled to assure proper ratio of anticoagulant to blood. They must be inverted immediately after filling to prevent clotting.
What is the light green tube used for in phlebotomy?
Light green-top tube (lithium heparin)
This tube contains lithium heparin and gel separator used for the collection of heparinized plasma for routine chemistry tests. NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen.
What tube is responsible for coagulation testing?
The specimen of choice for coagulation testing is plasma. Venous blood is drawn into a 3.2% buffered sodium citrate tube (blue top tube), yielding a whole blood sample with a 9:1 blood to anticoagulant ratio. Inadequate filling of the collection tube will decrease this ratio, and may affect test results.
Which tube is used for coagulation?
A light-blue top tube (containing 3.2% sodium citrate) that will be used for coagulation testing must be filled to completion. Please see the attached BD guides for instructions. Under-filling the tube changes the ratio of blood to anticoagulant additive. The under-filled tube will be considered an improper specimen.
What color tubes are used for which tests in phlebotomy?
PHLEBOTOMY SERVICES
| STOPPER COLOR | CONTENTS | USES/COMMENTS |
|---|---|---|
| Green | Sodium heparin(100 USP Units) | Ammonia, Lactate, HLA Typing |
| Tan | K2 EDTA | Lead levels |
| Yellow | ACD Solution A consists of trisodium citrate, citric acid and dextrose | DNA Studies, HIV Cultures |
| Pink | (K2)EDTA | Blood type & Screen, Compatibility Study, Direct Coombs HIV Viral Load |
Which collection tube must be completely filled for testing to occur?
What are light blue tubes used for? Coagulation! These contain sodium citrate, an anti-coagulant that binds calcium. These tubes MUST be filled completely.
What happens if coagulation specimen collection tube is underfilled?
Underfilled Coagulation Tubes
Calcium is essential for blood clotting. … If samples are under-filled, the citrate to blood ratio is too high and therefore, depending on how short, the added calcium will take a time to reverse the citrate effect leading to increased clotting times.
Which tube would a phlebotomist used to collect blood for type and screen?
K2 EDTA tube
| TYPE AND SCREEN | |
|---|---|
| ORDERING INFORMATION: | Geisinger Epic Procedure Code: LAB3207 Geisinger Epic ID: 15475 |
| SPECIMEN COLLECTION | |
| Specimen type: | Whole Blood |
| Preferred collection container: | 6 mL pink-top K2 EDTA tube |
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Nov 18, 2020
What tests go in what color tubes?
Blood Collection Tubes
| Tube cap color | Additive | Common laboratory tests |
|---|---|---|
| Lavender or pink | Potassium EDTA | Hematology and blood bank |
| Gray | Sodium fluoride, and sodium or potassium oxalate | Glucose (especially when testing will be delayed), blood alcohol, lactic acid |
Where are phlebotomy needles discarded after a venipuncture?
1. Properly dispose of the entire single draw apparatus and needle using the designated container on the phlebotomy tray or designated biohazard containers either on the patient’s counter or wall.