How big is mcherry

GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel is necessary for its fluorescence and therefore GFP cannot be downsized by deleting residues.

Far-red fluorescent proteins are beneficial for imaging in mammals. Here, starting from mCherry, the most commonly used among the different types of red fluorescent proteins (RFP), not having a H-bond network in its original form, we sought to recover the hydrogen bond network in mCherry.

mCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer with low acid sensitivity.

It’ll depend on the flow cytometer you have access to but to best detect the mCherry signal excite with the yellow-green laser at 561 nm and detect in the PE-TexasRed channel with a 610/20 bandpass filter.

The tandem dimer tdTomato is equally photostable but twice the molecular weight of mCherry, and may be used when fusion tag size does not interfere with protein function. mStrawberry is the brightest red monomer, but it is less photostable than mCherry, and should be avoided when photostability is critical.

mNeonGreen: a yellow-green fluorescent protein

mNeonGreen was reported as the brightest monomeric green or yellow fluorescent protein at the time. It is 1.5 to 3 times brighter than the most commonly used GFPs and YFPs.

The 561-nm (yellow-green) laser results in more efficient excitation (64%) of mCherry because this wavelength is closer to the maximum excitation wavelength than the 488-nm (blue) laser. The 561-nm laser therefore results in a brighter emission signal as shown in Figure 6.

To characterize cell and tissue dynamics during the formation of the cardiovascular system in mice, we generated a novel transgenic mouse line, Tg(Flk1∷myr-mCherry), in which endothelial cell membranes are brightly labeled with mCherry, a red fluorescent protein.